Whole-cell Detectors of Contaminants Constructed by Immobilization of Bioreporters in Form of Biofilm on Special Optical Fiber Elements


Jakub Zajic, Steven Ripp, Josef Trögl, Gabriela Kuncová, Marie Pospísilová


A whole-cell optical fiber sensor of toluene was prepared by the adsorption of Pseudomonas putida TVA8, the bacterial strain producing light in contact with toluene, in the form of biofilm. Bioreporter immobilization was facilitated by (3-Aminopropyl)triethoxysilane (APTES) treatment on the wider end of tapered quartz optical fiber element (OFE). Repeatability of preparation and response of the active part of the biosensor was demonstrated in five trials with different OFE geometries in which the bioluminescence (BL) was induced with toluene solution (26.5 mg L -1 ) for 2 weeks. Longevity of inductions of the biosensor was shown in over 135 days trial, when the biosensor was repetitively induced with toluene solution (26.5 mg L -1 ) 68 times. The intensities of BL gradually decreased due to release of the adsorbed cells and they were only temporarily restored by the addition of nutrients. The intensities of BL induced with contaminated ground water were lower than in the mineral medium (MSM) with the same content of toluene. Ideal shape of OFE, which maximizes the detected signal, was calculated to be a Frustrum cone. The developed sensor is plausible for multiple and fast online monitoring of the presence of a pollutant in remote localities but not its concentration.

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Zajic J, Ripp S, Trogl J, Kuncova G, Pospisilova M. 2019. Whole-cell detectors of contaminants constructed by immobilization of bioreporters in form of biofilm on special optical fiber elements, 2019 IEEE International Conference on Sensors and Nanotechnology, p. 1-4, Penang, Malaysia.