Michalsen, M. M., F. Kara Murdoch, F. E. Löffler, J. Wilson, P. B. Hatzinger, J. D. Istok, L. Mullins, A. Hill, R. W. Murdoch, C. Condee and K. H. Kucharzyk
Quantitative measurement of process-specific biomarker genes of Dehalococcoides mccartyi (Dhc) supports monitoring at chlorinated ethene contaminated sites. In this study, we varied Dhc cell abundances from ∼103 to 108 cells/mL in aquifer microcosms and correlated the corresponding reductive dehalogenase (RDase) gene and RDase protein abundances with measured reductive dechlorination (RD) rates of cis-1,2-dichloroethene (cDCE) and vinyl chloride (VC). An additional set of microcosms tested the RD rate-predictive power of the regression analyses. These efforts revealed (1) that targeted proteomics quantifies Dhc biomarker proteins (e.g., TceA and VcrA, OmeA) over a relevant range of Dhc cell densities, and (2) that protein and gene abundances can predict RD rates. Protein detection limits translated to a rate coefficient of 10–4 day–1 (0.04 year–1) for both kcDCE and kvc, which is within the range observed at sites undergoing monitored natural attenuation (MNA) (i.e., without the implementation of enhanced bioremediation treatment). Rates predicted using a combination of quantitative biomarker gene and protein measurements generally resulted in the best match with experimentally determined rate constants. These new findings provide evidence that quantitative biomarker measurements may be useful predictors of in situ RD rates, which would constitute a major advance for the cost-effective management of contaminated sites.
Michalsen, M. M., F. Kara Murdoch, F. E. Löffler, J. Wilson, P. B. Hatzinger, J. D. Istok, L. Mullins, A. Hill, R. W. Murdoch, C. Condee and K. H. Kucharzyk (2021). “Quantitative Proteomics and Quantitative PCR as Predictors of cis– ,2-Dichlorethene and Vinyl Chloride Reductive Dechlorination Rates in Bioaugmented Aquifer Microcosms.” ACS ES&T Engineering.