Nitrous Oxide Reduction Kinetics Distinguish Bacteria Harboring Clade I nosZ from those Harboring Clade II nosZ


Sukhwan Yoo, Silke Nissen, Doyoung Park, Robert A. Sanford, Frank E. Löffler


Bacteria capable of reduction of nitrous oxide (N2O) to N2 separate into clade I and clade II organisms on the basis of nos operon structures and nosZ sequence features. To explore the possible ecological consequences of distinct nos clusters, the growth of bacterial isolates with either clade I (Pseudomonas stutzeri strain DCP-Ps1, Shewanella loihica strain PV-4) or clade II (Dechloromonas aromatica strain RCB, Anaeromyxobacter dehalogenans strain 2CP-C) nosZ with N2O was examined. Growth curves did not reveal trends distinguishing the clade I and clade II organisms tested; however, the growth yields of clade II organisms exceeded those of clade I organisms by 1.5- to 1.8-fold. Further, whole-cell half-saturation constants (Kss) for N2O distinguished clade I from clade II organisms. The apparent Ks values of 0.324 ± 0.078 μM for D. aromatica and 1.34 ± 0.35 μM for A. dehalogenans were significantly lower than the values measured for P. stutzeri (35.5 ± 9.3 μM) and S. loihica (7.07 ± 1.13 μM). Gen

Access Full Publication


Yoon S, Nissen S, Park D, Sanford RA, Loffler FE. 2016. Nitrous oxide reduction kinetics distinguish bacteria harboring clade I nosZ from those harboring clade II nosZ. Applied and Environmental Microbiology 82:3793-3800.