Rebekah C. Kennedy, Russell R. Fling, Paul D. Terry, Fu-Min Mann, Jiangang Chen, Christopher J. Borman
Triclocarban (3,4,4′-Trichlorocarbanilide; TCC) in the environment has been well documented. Methods have been developed to monitor TCC levels from various matrices including water, sediment, biosolids, plants, blood and urine; however, no method has been developed to document the concentration of TCC in fecal content after oral exposure in animal studies. In the present study, we developed and validated a method that uses liquid extraction coupled with HPLC-MS/MS determination to measure TCC in feces. The limit of detection and limit of quantitation in control rats without TCC exposure was 69.0 ng/g and 92.9 ng/g of feces, respectively. The base levels of TCC in feces were lower than LOD. At 12 days of treatment, the fecal TCC concentration increased to 2220 µg/g among 0.2% w/w exposed animals. The concentration in fecal samples decreased over the washout period in 0.2% w/w treated animals to 0.399 µ/g feces after exposure was removed for 28 days. This method required a small amount of sample (0.1 g) with simple sample preparation. Given its sensitivity and efficiency, this method may be useful for monitoring TCC exposure in toxicological studies of animals.
Kennedy RC, Fling RR, Terry PD, Menn FM, Chen JG, Borman CJ. 2015. Extraction of 3,4,4 ‘-trichlorocarbanilide from rat fecal samples for determination by high pressure liquid chromatography-tandem mass spectrometry. International Journal of Environmental Research and Public Health 12:8125-8132.