Researchers:
Rebekah C. Kennedy, Russell R. Fling, Paul D. Terry, Fu-Min Mann, Jiangang Chen, Christopher J. Borman
Abstract:
Triclocarban (3,4,4′-Trichlorocarbanilide; TCC) in the environment has been well documented. Methods have been developed to monitor TCC levels from various matrices including water, sediment, biosolids, plants, blood and urine; however, no method has been developed to document the concentration of TCC in fecal content after oral exposure in animal studies. In the present study, we developed and validated a method that uses liquid extraction coupled with HPLC-MS/MS determination to measure TCC in feces. The limit of detection and limit of quantitation in control rats without TCC exposure was 69.0 ng/g and 92.9 ng/g of feces, respectively. The base levels of TCC in feces were lower than LOD. At 12 days of treatment, the fecal TCC concentration increased to 2220 µg/g among 0.2% w/w exposed animals. The concentration in fecal samples decreased over the washout period in 0.2% w/w treated animals to 0.399 µ/g feces after exposure was removed for 28 days. This method required a small amount of sample (0.1 g) with simple sample preparation. Given its sensitivity and efficiency, this method may be useful for monitoring TCC exposure in toxicological studies of animals.
Citation:
Kennedy RC, Fling RR, Terry PD, Menn FM, Chen JG, Borman CJ. 2015. Extraction of 3,4,4 ‘-trichlorocarbanilide from rat fecal samples for determination by high pressure liquid chromatography-tandem mass spectrometry. International Journal of Environmental Research and Public Health 12:8125-8132.
