Expression of a Humanized Viral 2A-mediated Lux Operon Efficiently Generates Autonomous Bioluminescence in Human Cells


Tingting Xu, Steven Ripp, Gary S. Sadler, Dan M. Close


Expression of autonomous bioluminescence from human cells was previously reported to be impossible, suggesting that all bioluminescent-based mammalian reporter systems must therefore require application of a potentially influential chemical substrate. While this was disproven when the bacterial luciferase (lux) cassette was demonstrated to function in a human cell, its expression required multiple genetic constructs, was functional in only a single cell type, and generated a significantly reduced signal compared to substrate-requiring systems. Here we investigate the use of a humanized, viral 2A-linked lux genetic architecture for the efficient introduction of an autobioluminescent phenotype across a variety of human cell lines.

Access Full Publication


Xu T, Ripp S, Sayler G, Close D. 2014. Expression of a humanized viral 2A-mediated lux operon efficiently generates autonomous bioluminescence in human cells. PLoS ONE 9:e96347.