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Next-Gen Illumina Sequencing

Next Gen Illumina Sequencing

The UT Genomics Core provides high-throughput next-generation sequencing services to all interested on-campus and off-campus researchers. Sequencing is performed using the Illumina MiSeq instrument located on the seventh floor of the Science and Engineering Research Facility (SERF) within the Center for Environmental Biotechnology (CEB).

The Illumina MiSeq platform can yield up to 15 Gb of DNA sequence in a single run. Applications include genome sequencing, RNA sequencing, metagenomics, and 16S metabarcoding, as well as custom amplicon sequencing.  Users may build their own libraries or Genomics Core staff can assist with library preparation.  Students are encouraged to participate in lab work associated with their individual projects.

The prices listed below are subject to change as material costs change.  Troubleshooting may necessitate additional costs.

For further information on applications, project design, and pricing, please contact Veronica Brown at GenomicsCore@utk.edu or 865-974-8080.  Please contact Core staff prior to beginning a project to ensure compatibility with the Illumina MiSeq.

 

The appropriate Illumina MiSeq flow cells for your project is determined by the depth of coverage needed, as well as the desired read length.  Contact Core facility staff for advice on which flow cell is right for your project. The costs below are for a PREPARED library.  Costs for library prep done at the Core facility are listed below and are in addition to the cost of the flow cell.

  Internal University of Tennessee user External academic user External non-academic user
Version 3, 600 cycle (2 X 300) kit; 13-15 Gb data, ~22-24 million reads $1,900.00 $2,023.00 $3,135.00
Version 3, 150 cycle (2 X 75) kit; 2-3 Gb data, ~22-24 million reads $1,305.00 $1,426.00 $2,153.00
Version 2, 500 cycle (2 X 250) kit; ~7-8 Gb data, ~12-14 million reads $1,560.00 $1,686.00 $2,574.00
Version 2, 300 cycle (2 x 150) kit; ~4-5 Gb data, ~12-14 million reads $1,440.00 $1,567.00 $2,376.00
Version 2, 50 cycle (2 X 25) kit; 750-850 MB data, ~12-14 million reads $1,230.00 $1,361.00 $2,030.00
MiSeq run only $398.00 $521.00 $657.00

 

Genome and metagenome libraries are prepared using Illumina’s Nextera XT.

  Internal University of Tennessee user External academic user external non-academic user
Nextera XT library Prep, first sample $321.00 $405.00 $530.00
Nextera XT library Prep, each additional sample up to 10 more $55.00 $61.25 $91.00

 

Amplicon libraries are prepared with Illumina’s Nextera XT amplicon protocol.  Users can prepare their own PCR product or Core staff can fully prepare the amplicon libraries with common primers.  Please contact Core staff prior to preparing your own library to confirm compatibility with the Illumina MiSeq.

  Internal University of Tennessee user External academic user external non-academic user
Full Amplicon library prep, up to 23 samples $1,191.00 $1,514.00 $1,965.00
Full Amplicon library prep, 24-47 samples $1,510.00 $1,876.00 $2,492.00
Full Amplicon library prep, 48-96 samples $1,982.00 $2,399.00 $3,270.00
Amplicon library prep, up to 23 samples $941.00 $1,150.00 $1,553.00
Amplicon library prep,  24-47 samples $1,172.00 $1,441.00 $1,934.00
Amplicon library prep,  48-96 samples $1,571.00 $1,853.00 $2,592.00

 

RNA libraries are prepared using Illumina’s TruSeq protocol.

  Internal University of Tennessee user External academic user external non-academic user
TruSeq mRNA Library Prep, one sample $865.00 $1,161.00 $1,427.00
TruSeq mRNA Library Prep, each additional sample up to 23 more $102.00 $116.00 $168.00
RiboZero RNA sample preparation, up to 6 samples $775.00 $861.00 $1,279.00

 

The Genomics Core houses additional equipment available for use.  The Pippin Prep performs automated gel extraction.  The Bioruptor uses ultrasound to fragment DNA.  The Bioanalzyer is used to quantify DNA and RNA.

  Internal University of Tennessee user External academic user external non-academic user
Pippin Prep, unassisteda $64.00 $97.00 $106.00
Pippin Prep, assistedb $134.00 $202.00 $221.00
Bioruptor, unassisteda $27.00 $41.00 $45.00
Bioruptor, assistedb $55.00 $83.00 $91.00
Bioanalyzer, High Sensitivity chips $108.00 $130.00 $178.00
Bioanalyzer, standard sensitivity chips $78.00 $98.00 $129.00

aUnassisted use implies that the user has been previously trained on the instrument and is capable of performing all needed tasks without assistance from Core facility personnel

bAssisted use implies that the user has not received instrument training and will require assistance from Core facility personnel for sample analysis

 

For assistance with Illumina data processing:

The CEB Bioinformatics Resource Center (BRC) provides access to computational resources and expert advice for assembling and analyzing Illumina data.  The BRC is located in SERF 736.

 

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