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Next-Gen Illumina Sequencing

The CEB provides high-throughput next-generation sequencing services to all interested on-campus and off-campus researchers. Sequencing is performed using the Illumina MiSeq instrument located on the seventh floor of the Science and Engineering Research Facility (SERF).

The Illumina MiSeq platform can yield up to 15 Gb of DNA sequence in a single run. Applications include genome sequencing, RNA sequencing, metagenomics, and 16S metabarcoding, as well as custom amplicon sequencing.  Users may build their own libraries or Genomics Core staff can assist with library preparation.  Students are encouraged to participate in the lab work associated with their individual projects.

For further information on applications, project design, and pricing, please contact Veronica Brown at GenomicsCore@utk.edu or 865-974-8080.

Sequencing PREPARED Libraries on Illumina’s MiSeq

MiSeq Sequencing kit Approximate output UT-clients Non-UT Academic Commercial
version 2, 50 cycle (2×25) 750-850 MB $1230 $1280 $1380
version 2, 300 cycle (2×150) 4-5 Gb $1440 $1490 $1590
version 2, 500 cycle (2×250) 7-8 Gb $1560 $1610 $1710
version 3, 150 cycle (2×75) 2-3 Gb $1305 $1355 $1455
version 3, 600 cycle (2×300) 13-15 Gb $1900 $1950 $2050

Note: prices subject to change

 

For assistance with Illumina data processing:

The CEB Bioinformatics Resource Center (BRC) provides access to computational resources and expert advice for assembling and analyzing Illumina data.  The BRC is located in SERF 736.

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