Co-Extraction of DNA and PLFA from Soil Samples


Sheridan Brewer, Stephen M. Techtmann, Nagissa Mahmoudi, Dijibril Niang, Susan Pfiffner, Terry C. Hazen


Lipid/DNA co-extraction from one sample is attractive in limiting biases associated with microbial community analysis from separate extractions. We sought to enhance established co-extraction methods and use high-throughput 16S rRNA sequencing to identify preferentially extracted taxa from co-extracted DNA. Co-extraction results in low DNA yields and distinct community structure changes.

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Brewer S, Techtmann SM, Mahmoudi N, Niang D, Pfiffner S, Hazen TC. 2015. Co-extraction of DNA and PLFA from soil samples. Journal of Microbiological Methods, doi:10.1016/j.mimet.2015.05.018.