The BD FACSAria flow cytometer/cell sorter is equipped with two lasers (Sapphire 488-50 Blue, COHR Cube 640-100 Red) to enable the detection of a variety of fluorochromes and fluorescent proteins. The optical configuration of this instrument is listed in the table below.
| Laser (Excitation) | Detector (Emission) | Fluorochrome examples |
| Blue, 488 nm | 780/60 | PE-Cy7 |
| 710/50 | PE-Cy5, PerCP, PerCP-Cy5.5 | |
| 575/25 | PE, PI | |
| 530/30 | FITC, Alexa Fluor 488, GFP | |
| Red, 640 nm | 780/60 | APC-Cy7, APC-H7 |
| 670/30 | APC, Alexa Fluor 647 |
In addition to flow cytometric analysis, the FACSAria can also perform 2-way, 4-way, and 96-well plate cell sorting.
Please click here for the full specifications of the BD FACSAria.
Please click here to learn more about flow cytometry and cell sorting.
Please contact Dr. Larry Millet (E-mail: lmillet1@utk.edu or 865-974-8369) if you need to use the FACSAria instrument, if you would like to be trained on the instrument, or if you have any questions.