A Real-time Non-invasive Auto-bioluminescent Urinary Bladder Cancer Xenograft Model


Bincy Anu John, Tingting Xu, Steven Rip, Hwa-Chain Robert Wang


Purpose: The study was to develop an auto-bioluminescent urinary bladder cancer (UBC) xenograft animal model for pre-clinical research.

Procedure: The study used a humanized, bacteria-originated lux reporter system consisting of six (luxCDABEfrp) genes to express components required for producing bioluminescent signals in human UBC J82, J82-Ras, and SW780 cells without exogenous substrates. Immune-deficient nude mice were inoculated with Lux-expressing UBC cells to develop auto-bioluminescent xenograft tumors that were monitored by imaging and physical examination.

Results: Lux-expressing auto-bioluminescent J82-Lux, J82-Ras-Lux, and SW780-Lux cell lines were established. Xenograft tumors derived from tumorigenic Lux-expressing auto-bioluminescent J82-Ras-Lux cells allowed a serial, non-invasive, real-time monitoring by imaging of tumor development prior to the presence of palpable tumors in animals.

Conclusions: Using Lux-expressing auto-bioluminescent tumorigenic cells enabled us to monitor the entire course of xenograft tumor development through tumor cell implantation, adaptation, and growth to visible/palpable tumors in animals.

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John BA, Xu T, Ripp S, Wang H-CR. 2017. A real-time non-invasive auto-bioluminescent urinary bladder cancer xenograft model. Molecular Imaging and Biology doi 10.1007/s11307-016-0989-y.